Together with our latest publication in Current Biology (Publication metrics and success on the academic job market) we launched a web tool that permits any grad student or postdoc in the life sciences to calculate his or her likelihood of becoming a principal investigator (PI).
Article on my collaboration with Eran Segal during my PhD, funded in part by an NBIC Fellowship
Research highlight of our noise paper
Marathon runners represent the dynamics of the transcription process, influenced by DNA sequence elements. In this issue, single-cell time-lapse microscopy experiments were used to shed light on the ways in which two types of promoter elements—nucleosome disfavoring sequences and transcription factor binding sites—influence transcription dynamics. Promoter dynamics dictate the transcriptional noise across a cell population, as illustrated by the variability between the runners.
Our new noise paper is featured on SGD’s ‘New & Noteworthy’
Our new noise paper just got published. In this work we explore the effects of two types of promoter elements on transcription: transcription factor binding sites and nucleosome disfavouring sequences. We show that both can change the mean expression of a gene, across a population of cells, however in different ways. Gene expression occurs in bursts, and we predict that changes in binding affinity change the number of transcripts produced per burst, while changes in the nucleosome occupancy change the frequency of bursts. The former should increase the noise with expression, the latter should decrease the noise. Both time-lapse microscopy and flow-cytometry measurements confirm this prediction.
Two DNA-encoded strategies for increasing expression with opposing effects on promoter dynamics and transcriptional noise
M Dadiani, D van Dijk, B Segal, Y Field, G Ben-Artzi, T Raveh-Sadka, M Levo, I Kaplow, A Weinberger and E Segal